Amyloid Proteins: Methods and Protocols

Part I. In Vivtro Models and Assays

1. Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-redox Cycling

Adam P. Gunn, Blaine R. Roberts and Ashley I Bush

2. Applications of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins

Dahabada H.J. Lopes, Sharmisthia Sinha, Clark Rosenweig, Gal Bitan

3. Preparation of Stable Amyloid β-Protein Oligomers of Defined Assembly Order

Clark Rosenweig, Kenjiro One, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow

4. Purifications and Fibrillation of Full-Length Recombinant PrP

Natallia Makarava and Ilia V. Baskakov

5. Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies

Miguel Calero and Maria Gasset

6. Quasielestic Light Scattering Study of Amyloid β-Protein Fibrillogenesis

Aleksey Lomakin and David B. Teplow

7. Conformations of Microtuble-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer (FRET)

Sadasivam Jeganathan, Subashchandrabrose Chinnathamb, Eva-Maria Mandelkow, Echard Mandelkow

8. Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM)

Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland

9. X-ray Fibre Diffraction Studies of Amyloid Fibrils

Kyle L Morris and Louise C Serpell

10.Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering

Annette Eva Langkilde and Bente Vestergaard

11.Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates

12. Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy

Susanne Wegmann, Daniel J. Muller and Eckhard Mandelkow

13. Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR

Mar?al Vilar, Lei Wang and Roland Riek

14.Cyclic Amplification of Prion Protein Misfolding

Marcelo A Barria, Dennisse Gonzalez-Romero, and Claudio Soto

15. Search for Amyloid Binding Proteins by Affinity Chromatography

Miguel Calero, Agueda Rostagno, and Jorge Ghiso

Part II Cell Culture Models and Assays

16. Using Biophysical Approaches for Establishing the Links Between Aβ Aggregation and Cytotoxicity in Vitro

Asad Jan and Hilal A. Lashuel

17. Preparation of Cultured Human Vascular Cells

Ingvar H. Olafsson, Dadi Th. Vilhjalmsson and Finnbogi R. Thormodsson

18. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse Brain

Sebastien A. Gauthier, Susmita Sahoo, Sonia S. Jung, and Efrat Levy

19. In Vitro Assays Measuring Protection by Proteins such as Cystatin C of Primary Cortical Neuronal and Smooth Muscle Cells

Sebastein A. Gauthier, Belen Tizon, Susmita Sahoo, and Efrat Levy

20. Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids

Carlos Villalobos, Erica Caballero, Sara Sanz-Blasco and Luc?a N??ez

21. Bacterial Amyloids

Yizhou Zhou, Luz Blanco, Daniel R. Smith, Matthew R. Chapman

22. Study of Amyloids using Yeast

Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey and Herman K. Edskes

23. Cell-to-cell Transmission of a-synuclein Aggregate

Seung-Jae Lee, Paula Desplats, He-Jin Lee, Brian Spencer and Eliezer Masliah

Part III In Vivo Models and Assays

24. Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies

Per Westermark

25. Analysis of S100 Oligomers and Amyloids

Hugo M. Botelho, G?nter Fritz, Cl?udio M. Gomes

26. S100A8/A9 Amyloidosis in the Ageing Prostate: Relating ex vivo and in vitro Studies

Anna L. Gharibyan, Dina Raveh, Ludmilla A.